Splicing Factor Interactions in Drosophila melanogaster - Appendices A: Synthetic Lethal Data

Appendices A: Synthetic Lethal Data

Table 1: Synthetic Lethal Fly Counts for Minutes and Genes Crossed with ry ED 195.
Number of flies observed is listed in parentheses below the genotypic class. Percent viability was determined by dividing the number of progeny of the significant genotype (the 1st column) by the number of progeny whose genotype was the most abundant ED = B52^ED allele; 195 = Ubx195

Genotype of	Number and Genotype of Progeny				Percent
Second Parent	of Crosses with ry ED 195	Viability
+	ry ED 195 ;	MKRS
+	+	+			69
	(36)	(52)
	M(1)18C[1] ;	M(1)18C[1] ;	FM6 ;	FM6 ;
M(1)18C[1]	+	+	+	+
FM6	ry ED 195	MKRS	ry ED 195	MKRS	5
	+	+	+	+
	(1)	(2)	(21)	(21)
	M(2)31A[3] ;	M(2)31A[3] ;	Cy ;	Cy ;
M(2)31A[3]	+	+	+	+
Cy	ry ED 195	MKRS	ry ED 195	MKRS	0
	+	+	+	+
	(0)	(7)	(17)	(19)
	M(2)41A[1] ;	M(2)41A[1] ;	SM2 ;	SM2 ;
M(2)41A[1]	+	+	+	+
SM2	ry ED 195	MKRS	ry ED 195	MKRS	53
	+	+	+	+
	(9)	(16)	(17)	(11)
	M(2)53[1] ;	M(2)53[1] ;	SM5 ;	SM5 ;
M(2)53[1]	+	+	+	+
SM5	ry ED 195	MKRS	ry ED 195	MKRS	100
	+	+	+	+
	(4)	(0)	(4)	(3)
	M(2)24F[1] ;	M(2)24F[1] ;	SM5 ;	SM5 ;
M(2)24F[1]	+	+	+	+
SM5	ry ED 195	MKRS	ry ED 195	MKRS	22
	+	+	+	+
	(7)	(17)	(18)	(32)
M(3)96C[2]	M(3)96C[2]	M(3)96C[2]	Cy	Cy
Cy	ry ED 195	MKRS	ry ED 195	MKRS	100
	(27)	(4)	(9)	(13)
M(3)65F[1]	M(3)65F[1]	M(3)65F[1]	Me[1]	Me[1]
Me[1]	ry ED 195	MKRS	ry ED 195	MKRS	0
	(0)	(3)	(27)	(30)
M(3)76A[1]	M(3)76A[1]	M(3)76A[1]	Me	Me
Me	ry ED 195	MKRS	ry ED 195	MKRS	91
	(10)	(0)	(11)	(0)
M(3)S36[1]	M(3)S36[1]	M(3)S36[1]	Me	Me
Me	ry ED 195	MKRS	ry ED 195	MKRS	100
	(11)	(11)	(11)	(0)
	M(3)X[1]	M(3)X[1]
M(3)X[1]	ry ED 195	MKRS			100
	(18)	(17)
M(3)80[1]	M(3)80[1]	M(3)80[1]	TM3	TM3
TM3	ry ED 195	MKRS	ry ED 195	MKRS	100
	(19)	Total for these two: (26)		(0)
M(3)95A[2]	M(3)95A2	M(3)95A2	TM2	TM2
TM2	ry ED 195	MKRS	ry ED 195	MKRS	0
	(0)	(0)	Total for these two : (22)
	M(2)39F[1]	M(2)39F[1]	SM5	SM5
M(2)39F[1]	+	+	+	+
SM5	ry ED 195	MKRS	ry ED 195	MKRS	27
	+	+	+	+
	(3)	(7)	(5)	(11)
	l(2)E151*	l(2)E151*	CyO	CyO
l(2)E151*	+	+	+	+
CyO	ry ED 195	MKRS	ry ED 195	MKRS	20
	+	+	+	+
	(4)	(20)	(19)	(14)
	M(2)36F[2?] ;	M(2)36F[2?] ;	Cy[1]cn[2];	Cy[1]cn[2];
	+	+	L[4]sp[2]	L[4]sp[2]
M(2)36F[2?]			+	+	74
Cy[1]cn[2];	ry ED 195	MKRS	ry ED 195	MKRS
L[4]sp[2]	+	+	+	+
	(17)	(42)	(22)	(23)
	M(2)60E[2] ;	M(2)60E[2] ;	Cy[1]cn[2] ;	Cy[1]cn[2] ;
M(2)60E[2]	+	+	+	+
Cy[1]cn[2]	ry ED 195	MKRS	ry ED 195	MKRS	59
	+	+	+	+
	(16)	(21)	(29)	(27)
	M(2)58F[1] ;	M(2)58F[1] ;	Sm[1]sp[2] ;	Sm[1]sp[2] ;
M(2)58F[1]	+	+	+	+
Sm[1]sp[2]	ry ED 195	MKRS	ry ED 195	MKRS	0
	+	+	+	+
	(0)	(21)	(16)	(19)
M(3)40130[1]	M(3)40130[1]	M(3)40130[1]	Dfd	Dfd
Dfd	ry ED 195	MKRS	ry ED 195	MKRS	100
	(20)	(18)	(0)	(0)
	M(2)31A[1] ;	M(2)31A[1] ;	Cy[1]cn[2]	Cy[1]cn[2]
M(2)31A[1]	+	+	lt[3]sp[2] ;	lt[3]sp[2] ;
Cy[1]cn[2];			+	+
lt[3]sp[2]	ry ED 195	MKRS	ry ED 195	MKRS	74
	+	+	+	+
	(20)	(27)	(6)	(13)
	w;1(2)49Db[TW6] ;	w;1(2)49Db[TW6] ;	CyO ;	CyO ;
w;1(2)49Db[TW6]	+	+	+	+
CyO	ry ED 195	MKRS	ry ED 195	MKRS	59
	+	+	+	+
	(13)	(25)	(3)	(26)
	fl(2)d[2]bw ;	fl(2)d[2]bw ;	CyO ;	CyO ;
fl(2)d[2]bw	+	+	+	+
CyO	ry ED 195	MKRS	ry ED 195	MKRS	80
	+	+	+	+
	(16)	(20)	(4)	(17)
	w;U2AF38[[Delta]E18] ;	w;U2AF38[[Delta]E18] ;	CyO ;	CyO ;
w;U2AF38[[Delta]E18]	+	+	+	+
CyO	ry ED 195	MKRS	ry ED 195	MKRS	37
	+	+	+	+
	(10)	(27)	(12)	(24)
	l(2)06694 ;	l(2)06694 ;	CyO ;	CyO ;
l(2)06694	+	+	+	+
CyO	ry ED 195	MKRS	ry ED 195	MKRS	40
	+	+	+	+
	(20)	(45)	(17)	(16)
	w; vir3 ;	w; vir3 ;	+ CyO ;	+ CyO ;
w; vir3	+	+	+	+
+ CyO	ry ED 195	MKRS	ry ED 195	MKRS	80
	+	+	+	+
	(24)	(27)	(22)	(30)
	U170K[1] ;	U170K[1] ;	CyO ;	CyO ;
U170K[1]	+	+	+	+
CyO	ry ED 195	MKRS	ry ED 195	MKRS	100
	+	+	+	+
	(28)	(25)	(13)	(20)
	U170K[62] ;	U170K[62] ;	CyO ;	CyO ;
U170K[62]	+	+	+	+
CyO	ry ED 195	MKRS	ry ED 195	MKRS	88
	+	+	+	+
	(21)	(24)	(23)	(13)
	y su(s)[R39] w ;	y su(s)[R39] w ;
y su(s)[R39] w	+	+
	ry ED 195	MKRS			91
	+	+
	(30)	(33)
	w[a]; su(w[a])[1] ;	w[a]; su(w[a])[1] ;
w[a]; su(w[a])[1]	+	+
	ry ED 195	MKRS			49
	+	+
	(19)	(39)
M(3)86D[1]	M(3)86D[1] ;	M(3)86D[1] ;	Me[2]Sb[sbd-1] ;	Me[2]Sb[sbd-1] ;
Me[2]Sb[sbd-1]	ry ED 195	MKRS	ry ED 195	MKRS	0
	(0)	(11)	(21)	(0)

Table 2: Synthetic lethal fly counts for Minutes and genes crossed with ry ED 9.22.
Number of flies observed is listed in parentheses below the genotypic class. ED = B52^ED allele; 9.22 = Ubx9.22

Genotype of	Number and Genotype of Progeny				Percent
Second Parent	of Crosses with ry ED 9.22	Viability
+	ry ED 9.22	MKRS
+	+	+			80
	(53)	(66)
	M(2)31A[3] ;	M(2)31A[3] ;	Cy ;	Cy ;
M(2)31A[3]	+	+	+	+
Cy	ry ED 9.22	MKRS	ry ED 9.22	MKRS	42
	+	+	+	+
	(5)	(12)	(11)	(10)
	M(2)41A[1] ;	M(2)41A[1] ;	SM2 ;	SM2 ;
M(2)41A[1]	+	+	+	+
SM2	ry ED 9.22	MKRS	ry ED 9.22	MKRS	64
	+	+	+	+
	(9)	(4)	(14)	(8)
	M(2)53[1] ;	M(2)53[1] ;	SM5 ;	SM5 ;
M(2)53[1]	+	+	+	+
SM5	ry ED 9.22	MKRS	ry ED 9.22	MKRS	48
	+	+	+	+
	(22)	(30)	(30)	(46)
	M(2)24F[1] ;	M(2)24F[1] ;	SM5 ;	SM5 ;
M(2)24F[1]	+	+	+	+
SM5	ry ED 9.22	MKRS	ry ED 9.22	MKRS	22
	+	+	+	+
	(5)	(15)	(7)	(23)
M(3)96C[2]	M(3)96C[2]	M(3)96C[2]	Cy	Cy
Cy	ry ED 9.22	MKRS	ry ED 9.22	MKRS	100
	(21)	(14)	(0)	(1)
M(3)65F[1]	M(3)65F[1]	M(3)65F[1]	Me[1]	Me[1]
Me[1]	ry ED 9.22	MKRS	ry ED 9.22	MKRS	0
	(0)	(11)	(8)	(25)
M(3)76A[1]	M(3)76A[1]	M(3)76A[1]	Me	Me
Me	ry ED 9.22	MKRS	ry ED 9.22	MKRS	100
	(17)	(0)	(8)	(0)
M(3)S36[1]	M(3)S36[1]	M(3)S36[1]	Me	Me
Me	ry ED 9.22	MKRS	ry ED 9.22	MKRS	100
	(17)	(14)	(8)	(0)
	M(3)X[1]	M(3)X[1]
M(3)X[1]	ry ED 9.22	MKRS			73
	(8)	(11)
M(3)80[1]	M(3)80[1]	M(3)80[1]	TM3	TM3
TM3	ry ED 9.22	MKRS	ry ED 9.22	MKRS	100
	(13)	Total for these two: (18)		()
	M(2)32A[1] ;	M(2)32A[1] ;	CyO ;	CyO ;
M(2)32A[1]	+	+	+	+
CyO	ry ED 9.22	MKRS	ry ED 9.22	MKRS	76
	+	+	+	+
	(13)	(12)	(8)	(17)
	l(2)E68M* ;	l(2)E68M* ;	CyO ;	CyO ;
l(2)E68M*	+	+	+	+
CyO	ry ED 9.22	MKRS	ry ED 9.22	MKRS	35
	+	+	+	+
	(7)	(16)	(20)	(19)
	M(2)36F[2?] ;	M(2)36F[2?] ;	SM5 ;	SM5 ;
M(2)36F[2?]	+	+	+	+
SM5	ry ED 9.22	MKRS	ry ED 9.22	MKRS	18
	+	+	+	+
	(4)	(22)	(9)	(20)
	M(2)39F[1] ;	M(2)39F[1] ;	SM5 ;	SM5 ;
M(2)39F[1]	+	+	+	+
SM5	ry ED 9.22	MKRS	ry ED 9.22	MKRS	27
	+	+	+	+
	(4)	(9)	(13)	(15)
	l(2)E151* ;	l(2)E151* ;	CyO ;	CyO ;
l(2)E151*	+	+	+	+
CyO	ry ED 9.22	MKRS	ry ED 9.22	MKRS	24
	+	+	+	+
	(4)	(5)	(15)	(17)
	M(2)36F[2?] ;	M(2)36F[2?] ;	Cy[1]cn[2]	Cy[1]cn[2]
	+	+	L[4]sp[2] ;	L[4]sp[2] ;
M(2)36F[2?]			+	+	81
Cy[1]cn[2];	ry ED 9.22	MKRS	ry ED 9.22	MKRS
L[4]sp[2]	+	+	+	+
	(29)	(36)	(22)	(27)
	M(2)60E[2] ;	M(2)60E[2] ;	Cy[1]cn[2] ;	Cy[1]cn[2] ;
M(2)60E[2]	+	+	+	+
Cy[1]cn[2]	ry ED 9.22	MKRS	ry ED 9.22	MKRS	64
	+	+	+	+
	(9)	(9)	(11)	(14)

	M(2)58F[1] ;	M(2)58F[1] ;	Sm[1]sp[2] ;	Sm[1]sp[2] ;
M(2)58F[1]	+	+	+	+
Sm[1]sp[2]	ry ED 9.22	MKRS	ry ED 9.22	MKRS	31
	+	+	+	+
	(8)	(14)	(22)	(26)
M(3)40130[1]	M(3)40130[1]	M(3)40130[1]	Dfd	Dfd
Dfd	ry ED 9.22	MKRS	ry ED 9.22	MKRS	0
	(0)	(16)	(14)	(9)
	M(2)31A[1] ;	M(2)31A[1] ;	Cy[1]cn[2]	Cy[1]cn[2]
M(2)31A[1]	+	+	lt[3]sp[2] ;	lt[3]sp[2] ;
Cy[1]cn[2];			+	+
lt[3]sp[2]	ry ED 9.22	MKRS	ry ED 9.22	MKRS	100
	+	+	+	+
	(21)	(13)	(17)	(13)
	y sn w	y sn w	FM7 ;	FM7 ;
	snf [J210] ;	snf [J210] ;	+	+
y sn w;	+	+			29
snf [J210]	ry ED 9.22	MKRS	ry ED 9.22	MKRS
FM7	+	+	+	+
	(5)	(14)	(17)	(3)
	w;1(2)49Db[TW6] ;	w;1(2)49Db[TW6] ;	CyO ;	CyO ;
w;1(2)49Db[TW6]	+	+	+	+
CyO	ry ED 9.22	MKRS	ry ED 9.22	MKRS	100
	+	+	+	+
	(46)	(42)	(29)	(29)
	fl(2)d[2]bw ;	fl(2)d[2]bw ;	CyO ;	CyO ;
fl(2)d[2]bw	+	+	+	+
CyO	ry ED 9.22	MKRS	ry ED 9.22	MKRS	46
	+	+	+	+
	(6)	(13)	(6)	(5)
	w U2AF38[[Delta]E18] ;	w U2AF38[[Delta]E18] ;	CyO ;	CyO ;
w;U2AF38[[Delta]E18]	+	+	+	+
CyO	ry ED 9.22	MKRS	ry ED 9.22	MKRS	87
	+	+	+	+
	(34)	(39)	(37)	(36)
	1(2)06694 ;	1(2)06694 ;	CyO ;	CyO ;
l(2)06694	+	+	+	+
CyO	ry ED 9.22	MKRS	ry ED 9.22	MKRS	11
	+	+	+	+
	(2)	(19)	(2)	(2)
	w; vir3 ;	w; vir3 ;	+ ; CyO ;	+ ; CyO ;
w; vir3	+	+	+	+
+ CyO	ry ED 9.22	MKRS	ry ED 9.22	MKRS	86
	+	+	+	+
	(31)	(36)	(35)	(36)
	U170K[1] ;	U170K[1] ;	CyO ;	CyO ;
U170K[1]	+	+	+	+
CyO	ry ED 9.22	MKRS	ry ED 9.22	MKRS	100
	+	+	+	+
	(25)	(22)	(13)	(18)
	U170K[62] ;	U170K[62] ;	CyO ;	CyO ;
U170K[62]	+	+	+	+
CyO	ry ED 9.22	MKRS	ry ED 9.22	MKRS	100
	+	+	+	+
	(19)	(16)	(16)	(15)
	y su(s)[R39] w ;	y su(s)[R39] w ;
y su(s)[R39] w	+	+
	ry ED 9.22	MKRS			100
	+	+
	(36)	(34)
	w[a]; su(w[a])[1] ;	w[a]; su(w[a])[1] ;
w[a]; su(w[a])[1]	+	+
	ry ED 9.22	MKRS			84
	+	+
	(26)	(31)
	M(3)86D[1] ;	M(3)86D[1] ;	Me[2]Sb[sbd-1] ;	Me[2]Sb[sbd-1] ;
M(3)86D[1]	+	+	+	+
Me[2]Sb[sbd-1]	ry ED 9.22	MKRS	ry ED 9.22	MKRS	43
	+	+	+	+
	(10)	(19)	(23)	(0)